Method and apparatus for microbiological analysis of...

C - Chemistry – Metallurgy – 12 – M

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C12M 1/34 (2006.01) C12Q 1/06 (2006.01) G01N 21/51 (2006.01)

Patent

CA 2093984

- 18 - ABSTRACT Method for microbiological analysis of biological samples in liquid suspension by light-scattering technique, which employs test-tube containers (11) made of a material transparent to electromagnetic radiations of a determined wave length, each container (11) cooperating with a thermostat system (17) and at least momentarily with a stirring assembly (31) and relative keeper stirring means (18), the container (11) cooperating with a focussing and collimation system (22) and with a monitoring device (24), the electromagnetic radiation of a determined wave length emitted with its own axis (29) of emission and transmitted through the container (11) by the focussing and collimation system (22) being scattered by the microorganisms present in the sample with an intensity related to their number and morphology, being this scattered radiation picked-up in succession with desired timing by the monitoring device (24) according to determined angles, from 0° to 180°, in relation to the emission axis (29) with a resulting data collection and construction of a curve, representing intensity of scattered radiation versus time, whose parameters are compared with reference values contained in a data bank of a data processing unit (13) for determination of the typical parameters of the analysis which are the counting, the replication rate and the morphology of microorganisms present in the sample. Apparatus for the microbiological analysis of biological samples in liquid suspension by light-scattering technique, which employs test-tube containers (11) made of a material transparent to electromagnetic radiations of a determined wave length and comprises a stand (12) with seatings (111) to position containers (11) with a substantially vertical axis and also a thermostat system (17), each container (11) - 19 - cooperating at least momentarily with a stirring assembly (31) that includes keeper means (18) located within the container (11), each container (11) cooperating with a reading means (26), which comprises a focussing and collimation system (22) having its axis of emission (29) substantially orthogonal to the vertical axis of the container (11) and also a monitoring device (24), the whole being governed by a data processing unit (13), the reading means (26) and the individual containers (11) being movable reciprocally with a position of reciprocal cooperation, the monitoring device (24) comprising at least two monitors (28) which, at least momentarily during the analysis, are positioned on a plane substantially orthogonal to the vertical axis of the container (11) and containing the axis (29) of emission and are also positioned at least at two fixed angles between 0° and 180° in relation to the axis (29) of emission.

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