Method and apparatus for patch-clamp measurements on cells

Details Method and apparatus for patch-clamp measurements on cells Method and apparatus for patch-clamp measurements on cells

G - Physics

01

N

G01N 33/487 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2417796

The invention shows a method for patch-clamp experiments on cells or similar structures, where at least one cell is inserted into the lumen of a capillary and is positioned inside the capillary so, that a sufficiently tight seal with a resistance exceeding 1, preferably 10 GigaOhm develops between cell membrane and inner surface of the capillary. Along its length said capillary has at least at one position a smaller inner diameter than the outer diameter of said cell. Preferably the cell is inserted and positioned in the capillary by pressure, suction, sedimentation or centrifugation of a suspension or solution containing said cell. An apparatus for performing experiments using this method is described.

L'invention concerne une technique patch-clamp permettant de pratiquer des expériences sur des cellules ou des structures similaires, au moins une cellule étant introduite dans la lumière d'un capillaire et positionnée à l'intérieur de celui-ci de sorte qu'une fermeture suffisamment étanche dont la résistance dépasse 1 et de préférence 10 GigaOhm se développe entre la membrane cellulaire et la surface intérieure dudit capillaire. Le capillaire présente sur sa longueur, au moins au niveau d'une position, un diamètre intérieur inférieur au diamètre extérieur de la cellule. La cellule est, de préférence, introduite et positionnée dans le capillaire par pression, aspiration, sédimentation, ou centrifugation d'une suspension ou d'une solution contenant ladite cellule. L'invention concerne également un appareil permettant d'effectuer des expériences au moyen de ladite technique.

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