Method and apparatus for performing large numbers of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2386791

The present invention relates to a method and apparatus for performing a large number of reactions using array assembly. In particular, the present invention features a method and apparatus for performing a large number of chemical and biological reactions by bringing two arrays into close apposition and allowing reactants on the surfaces of two arrays to come into contact. The present invention is exemplified by performing a large number of polynucleotide amplification reactions using array assembly. In addition, the present invention features a method and apparatus for coupling the amplification of polynucleotides and the detection of sequence variations, expression levels, and functions thereof.

La présente invention concerne un procédé et un appareil destinés à produire un grand nombre de réactions au moyen d'une plaque matrice. Cette invention concerne, en particulier, un procédé et un appareil destinés à produire un grand nombre de réactions chimiques et biologiques en plaçant deux plaques matrices en apposition et en permettant aux réactifs situés à la surface des ces deux plaques d'entrer en contact. L'invention est illustrée par la production d'un grand nombre de réactions inhérentes à l'amplification de polynucléotides au moyen d'une plaque matrice. En outre, la présente invention concerne un procédé et un appareil destinés à coupler l'amplification de polynucléotides, la détection de variations de séquences, de niveaux d'expression et de fonctions correspondantes.

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