Method and apparatus for performing submicroliter reactions...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) B01L 3/02 (2006.01) B01L 7/00 (2006.01) C12M 1/40 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) B01L 3/00 (2006.01)

Patent

CA 2474429

Methods for preparing nanoscale reactions using nucleic acids or proteins are presented, Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Proteins are captured specifically and saturably on the modified inner surface of the reaction chamber, typically a capillary. Excess protein is removed and the reaction is performed directly within the capillary. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput reactions involving nucleic acids or proteins are also provided.

La présente invention concerne des méthodes de préparation de réactions à l'échelle nanométrique à l'aide d'acides nucléiques ou de protéines. Lesdits acides nucléiques sont capturés de manière saturable, mais réversible, sur la surface intérieure de la chambre de réaction, généralement un capillaire. L'acide nucléique en excès est éliminé et la réaction est mise en oeuvre directement dans le capillaire. Des protéines sont capturées de manière spécifique et saturable sur la surface intérieure modifiée de la chambre de réaction, généralement un capillaire. Les protéines en excès sont éliminées et la réaction est mise en oeuvre directement dans le capillaire. L'invention concerne également des dispositifs de mise en oeuvre des méthodes de l'invention et un système conçu de manière avantageuse pour utiliser lesdites méthodes pour des réactions à rendement élevé impliquant des acides nucléiques ou des protéines.

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