Method and apparatus for producing platelet rich plasma...

A - Human Necessities – 61 – K

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A61K 35/16 (2006.01) A61K 35/14 (2006.01) B01D 21/26 (2006.01) B04B 5/02 (2006.01) B04B 11/00 (2006.01) B04B 15/00 (2006.01)

Patent

CA 2334887

Platelet rich plasma and/or platelet concentrate is prepared by placing whole blood in a first chamber (6) of a sterile processing disposable having two chambers (6, 8). The processing disposable is subjected to a first centrifugation to separate red blood cells (36), and the resulting platelet rich plasma supernatant (38) is decanted to the second chamber (8). The processing disposable is subjected to a second centrifugation to concentrate platelets. A volume of the platelet poor plasma supernatant in the second chamber (8) is removed, and the platelets are re-suspended in the remaining plasma. The second chamber (8) may contain anticoagulant (34) to preclude aggregation of the platelets.

L'invention concerne un plasma riche en plaquettes et/ou un concentré de plaquettes, que l'on prépare en plaçant du sang total dans la première chambre (6) d'un dispositif jetable de traitement stérile comprenant deux chambres (6, 8). On soumet alors le dispositif jetable de traitement à une première centrifugation visant à séparer les globules rouges (36), et on laisse décanter le surnageant (38) plasmatique riche en plaquettes ainsi obtenu dans la seconde chambre (8). On soumet ensuite le dispositif jetable de traitement à une seconde centrifugation visant à concentrer les plaquettes. On retire un certain volume du surnageant plasmatique pauvre en plaquettes de la seconde chambre (8), et les plaquettes sont remises en suspension dans le plasma restant. La seconde chambre (8) peut renfermer un anticoagulant (34) destiné à empêcher l'agrégation des plaquettes.

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