Method and apparatus for prokaryotic and eukaryotic cell...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/06 (2006.01) C12Q 1/04 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2427106

This invention describes methods and kits for quantifying viable cells in a sample using fluorescent dyes that can be internalized predominately by viable cells and have fluorescence properties measurably altered when bound to target components. These methods circumvent the need for training personnel in plating, growing and count viable cells, and reduce both the time and the cost required for cellular quantitation that use existing methods.

La présente invention décrit des méthodes et des kits de quantification de cellules viables dans un échantillon, utilisant des colorants fluorescents pouvant être internalisés principalement par des cellules viables et qui ont des propriétés de fluorescence modifiées de façon mesurables lorsqu'elles sont liées à des constituants cibles. Ces procédés permettent d'éviter d'avoir à former du personnel à la mise en culture, à la croissance et à la numération de cellules viables et permettent de réduire à la fois le temps et les coûts nécessaires à la quantification cellulaire utilisés par les méthodes actuelles.

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