Method and apparatus for purifying islets of langerhans

Details Method and apparatus for purifying islets of langerhans Method and apparatus for purifying islets of langerhans

C - Chemistry, Metallurgy

12

N

C12N 5/08 (2006.01) A61K 35/39 (2006.01) B01D 21/00 (2006.01) B01D 24/00 (2006.01) C12M 1/26 (2006.01)

Patent

CA 2091818

A method and apparatus to concentrate and purify islets of Langerhans from a tissue suspension containing islets and tissue fragments. The tissue suspension is flowed through an inclined channel such that laminar flow is established in the channel. The islets settle toward the channel bottom and are drawn out of the channel through an outlet in the channel bottom, while the remaining tissue suspension flows out a second outlet positioned higher than the islet outlet. Additional concentration or purification may be accomplished by passing the islets through the channel additional times and by centrifuging or filtering operations.

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