G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 27/447 (2006.01)
Patent
CA 2391302
Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.
L'invention concerne des procédés permettant de réduire une largeur de pic associée à l'établissement d'un champ d'électrophorèse dans un processus d'électrophorèse capillaire, et un appareil utile pour mettre en oeuvre lesdits procédés. Ces procédés consistent à définir une pente de progression maximum à utiliser pendant une progression initiale de champ électrique, à définir une période minimum pendant laquelle on établit le champ d'électrophorèse, et/ou à maintenir la température d'un milieu de séparation dans certaines plages pendant ladite progression initiale du champ électrique. L'invention concerne également des procédés permettant de déterminer la pente de progression permettant de réduire la largeur de pic de façon efficace.
Johnson Ben F.
Nordman Eric S.
Applied Biosystems Llc
Pe Corporation (ny)
Sim & Mcburney
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