Method and apparatus for screening microscopic cells...

G - Physics – 01 – N

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G01N 33/574 (2006.01) G01N 15/12 (2006.01) G01N 21/84 (2006.01) G01N 33/49 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2096530

ABSTRACT A method and apparatus for performing screening of one or more cell groups of interest obscured by a cell population utilizing at least one light sensing parameter. The cell group of interest such as a subset of a white blood cell population is enumerated by utilizing microspheres having monoclonal antibodies bound thereto to modify the sensed characteristics of specified cells to differentiate the cell group of interest from the obscuring cell population. A whole blood sample or portion thereof also can be measured first, then have specified cells deleted therefrom to enable the cell group of interest to be sensed in an area in which it would otherwise have been obscured by the deleted cells. The sample portion is again measured and compared to the first measurement to differentiate the cell group of interest.

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