Method and apparatus for screening obscured or partially...

Details Method and apparatus for screening obscured or partially... Method and apparatus for screening obscured or partially...

G - Physics

01

N

G01N 33/554 (2006.01) C12M 1/34 (2006.01) G01N 15/12 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/96 (2006.01) G01N 35/00 (2006.01) G01N 15/10 (2006.01)

Patent

CA 2081565

ABSTRACT OF THE DISCLOSURE A method and apparatus for screening obscured or partially obscured cells to enumerate one or more cell population subsets. A white blood cell population is counted, along with a standard population. The standard population can be one of the total number of white blood cell populations, a white blood cell population which does not obscure the shifted or non-shifted sensed characteristic of the subset of interest, an artificial population formed by microspheres which does not obscure the shifted or non-shifted sensed characteristic of the subset of interest or a white blood cell population into which the sensed characteristic of the subset will be wholly or partially shifted. The sensed characteristic of the subset of interest is then shifted by binding to microspheres having monoclonal antibodies specific to the subset of interest. The white blood cell population and the standard population are recounted and compared to the original counts.

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