Method and apparatus for separating biological materials and...

Details Method and apparatus for separating biological materials and... Method and apparatus for separating biological materials and...

G - Physics

01

N

G01N 15/06 (2006.01) B01L 99/00 (2010.01) A01N 1/02 (2006.01) B01D 3/00 (2006.01) B01D 11/00 (2006.01) B01D 35/06 (2006.01) B01J 19/08 (2006.01) B01L 3/00 (2006.01) B03C 1/01 (2006.01) B03C 1/02 (2006.01) C12M 1/00 (2006.01) C12M 1/40 (2006.01) G01N 21/00 (2006.01) G01N 21/76 (2006.01) G01N 21/77 (2006.01) G01N 33/531 (2006.01) G01N 33/538 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/553 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2358069

A method for separating biological materials and other substances is disclosed, wherein a mixture containing desired and undesired components are exposed to magnetic particles having ligands capable of binding to the desired and/or the undesired components to form a magnetic mixture, placing the magnetic mixture onto a substrate material; exposing the substrate coated with the magnetic mixture to a magnetic field of sufficient strength to cause the magnetic components to migrate across the substrate; and repeatedly increasing and decreasing the magnetic field in a pulsing manner with a frequency sufficient to cause the desired magnetic components to separate spatially from the undesired magnetic components. A device for separating biological materials capable of being operated to increase and decrease magnetic field in a pulse fashion is also disclosed.

La présente invention concerne une méthode visant à séparer des matières biologiques et autres substances. Un mélange, contenant des composants ciblés et des composants non ciblés, est exposé à des particules magnétiques ayant des ligands capables de se fixer sur les composants ciblés et/ou non ciblés pour former un mélange magnétique. Ledit mélange magnétique est placé sur un substrat, et ce dernier, recouvert par le mélange magnétique, est ensuite exposé à un champ magnétique suffisamment puissant pour faire migrer les composants magnétiques d'un côté à l'autre du substrat. Le champ magnétique est augmenté ou réduit à plusieurs reprises par impulsion, avec une fréquence suffisante pour permettre la séparation spatiale des composants magnétiques ciblés des composants magnétiques non ciblés. L'invention concerne également un dispositif permettant de séparer les matières biologiques et pouvant s'utiliser pour augmenter et réduire le champ magnétique par impulsion.

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