Method and apparatus for separating cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/00 (2006.01) C12M 1/00 (2006.01) C12M 1/42 (2006.01) C12N 13/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2599043

It is intended to provide a method of selectively separating target cells whereby non-target cells are selectively removed from a mass of cells consisting of cells in different types, and a cell separation apparatus. A method of selectively separating target cells which comprises locating a mass of cells consisting of cells in different types on a specific two-dimensional coordinate system either as individual cells or cell groups having two or more cells, distinguishing the thus located individual cells or cells in the cell groups depending on shape or size thereof or by using a cell marker, and irradiating the position or area wherein non-target cells or cell groups containing non-target cells exist with a physical energy typified by laser beams to thereby selectively kill the non-target cells or damage the function thereof, and an apparatus therefor.

L~invention concerne un procédé pour séparer sélectivement des cellules cibles. Le procédé de l'invention permet de retirer les cellules non-cibles d~une manière sélective d~une masse de cellules consistant en cellules de types différents, et un appareil de séparation cellulaire. Cette invention se rapporte à un procédé pour séparer sélectivement des cellules cibles qui comprend le repérage d~une masse de cellules consistant en cellules de types différents sur un système de coordonnées spécifique bidimensionnel soit en tant que cellules individuelles ou en tant que groupes cellulaires comportant deux ou plusieurs cellules, la distinction des cellules individuelles ainsi repérées ou des cellules dans les groupes cellulaires selon leur forme et leur taille à l~aide d~un marqueur cellulaire, et l~irradiation de la position ou de la zone selon lesquelles les cellules non-cibles ou les groupes cellulaires contenant des cellules non-cibles existent avec une énergie physique caractérisée par des rayons laser pour tuer ainsi sélectivement les cellules non-cibles ou endommager leurs fonctions, et un appareil fournit à ces fins.

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