B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
167/114, 128/63
B01D 21/26 (2006.01) A61K 35/18 (2006.01) B01D 43/00 (2006.01) G01N 33/49 (2006.01)
Patent
CA 1210693
ABSTRACT The present invention relates to apparatus and method for separating, by centrifugation, blood lymphocytes from other leuko- cytes in a blood sample. Small dense particles, either wetted or suspended in a physiological, hydrophilic medium, and a chemi- cally inert hydrophobic barrier material are initially located in a suitable container. When the blood sample is added to the container, the particles are phagocytized by the monocytes and granulocytes, so as to increase their specific gravities. Upon centrifugation, the barrier material, whose specific gravity is only slightly greater than the specific gravity of the lympho- cytes and less than the respective specific gravities of the phagocytic cells which have ingested particles and of the erythro- cytes (red blood cells) in the blood sample, forms a rigid barrier therebetween, allowing harvesting of the lymphocytes, substantially free of contamination.
445739
Macrae & Co.
Technicon Instruments Corporation
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