Method and apparatus for sorting cells

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/34 (2006.01) C12M 1/42 (2006.01) C12Q 1/00 (2006.01) G01N 15/10 (2006.01) G01N 15/14 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2728289

A method, apparatus, and system for a sorting flow cy-tometer include an objective lens having an optical axis coaxially aligned with the flow path at the focal point. A controllable energy source selec-tively alters an analyte according to a determination of whether the analyte is in a desired sub-population. In various embodiments, one or both of the emission from the controllable energy source and/or the emission from an illumination energy source passes through the objective lens. In some em-bodiments in which the emission from the controllable energy source passes through the objective lens, the objective lens may focus the emis-sion from the controllable energy source at a different point than the focal point of a signal detected from the analyte and, in particular, at a point closer to the objective lens.

La présente invention concerne un procédé, un appareil et un système pour un cytomètre de flux de tri comprenant une lentille dobjectif ayant un axe optique coaxialement aligné avec la voie de flux au niveau du point focal. Une source dénergie commandable de manière sélective modifie un mélange à analyser en fonction dune détermination de si oui ou non le mélange à analyser appartient à une sous-population souhaitée. Dans divers modes de réalisation, une émission ou les deux en provenance de la source dénergie commandable et/ou lémission provenant dune source dénergie déclairage traverse la lentille dobjectif. Dans certains modes de réalisation dans lesquels lémission en provenance de la source dénergie commandable traverse la lentille dobjectif, la lentille dobjectif peut cibler lémission provenant de la source dénergie commandable à un point différent du point focal dun signal détecté depuis le mélange à analyser et, notamment, à un point plus proche de la lentille dobjectif.

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