Method and apparatus for sterile cultivation of cells on...

Details Method and apparatus for sterile cultivation of cells on... Method and apparatus for sterile cultivation of cells on...

A - Human Necessities

01

G

195/145, 195/58

A01G 1/04 (2006.01)

Patent

CA 1125202

ABSTRACT OF THE INVENTION The invention disclosed comprises a process and apparatus for the cultivation of cells, and having particular application to the production of mushroom spawn, in which a particulate substrate, water and, in some cases, chalk are introduced into a rotary blender and thoroughly sized and sterilized at a temperature of about 250°F. The mixture is cooled in the blender and a cell line inoculum is sterilely introduced into the mixture and thoroughly blended with the substrate, The blended inoculum and substrate are transferred in a sterile air flow from the blender to sterile containers which include a breathing strip in which the cells lines are incubated. The apparatus includes the combination of blender, transfer means and a clean room for filling the sterile containers.

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