Method and apparatus for the detection of microorganisms

G - Physics – 01 – N

Patent

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Details

G01N 27/02 (2006.01) G01N 15/10 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2559393

The present application discloses a method for detecting a viable microorganism in a sample. The method may comprise (i) providing a detecting electrode and a counter electrode, (ii) contacting said sample with said detecting electrode and said counter electrode and (iii) measuring a difference in impedance between said detecting electrode and said counter electrode. The detecting electrode may comprise gold nanoparticles deposited thereon and/or a capture molecule. The capture molecule may be able to bind to the microorganism. A redox mediator can also be added to the sample prior to the impedance measurement. Also disclosed are related apparatuses and uses.

L'invention concerne un procédé servant à détecter un micro-organisme viable dans un spécimen. Ce procédé consiste (i) à mettre en application une électrode de détection et une contre-électrode, (ii) à mettre en contact ledit spécimen avec ladite électrode de détection et ladite contre-électrode et (iii) à mesurer une différence d'impédance entre ladite électrode de détection et ladite contre-électrode. L'électrode de détection peut comprendre des nanoparticules d'or déposées sur sa surface et/ou une molécule de capture. Cette dernière peut se fixer au micro-organisme. Il est également possible d'ajouter au spécimen un médiateur d'oxydoréduction préalablement à la mesure d'impédance. Elle concerne également des dispositifs et des utilisations associés.

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