Method and apparatus for the production of tgf-beta and...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/02 (2006.01) C07K 1/22 (2006.01) C07K 14/495 (2006.01) C12M 3/06 (2006.01)

Patent

CA 2066576

11972-42 METHOD AND APPARATUS FOR THE PRODUCTION OF TGF-B ABSTRACT OF THE DISCLOSURE Transforming growth factor B (TGF-B) is produced in relatively large quantities and at a relatively high purity by fermentation in a perfusion microcarrier reactor. Conditioned media from the reactor is first treated to provide the active form of TGF-B and subsequently purified by cation exchange chromatography followed by hydrophobic interaction chromatography. Optionally, nucleic acids complexed with the TGF-B may be removed while the protein is bound to the cation exchange resin. Purified TGF-Bl compositions have a specific activity above 107 U/ml. C:\WORK\PAT\11972421.4NO

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