Method and apparatus for three-dimensional microscopy with...

G - Physics – 02 – B

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G02B 21/22 (2006.01) G02B 21/36 (2006.01)

Patent

CA 2210801

A method and apparatus for three-dimensional optical microscopy is disclosed which employs dual opposing objective lenses (70, 72) about a sample (94) and extended incoherent illumination (84) to provide enhanced depth resolution. The observed light from both objective lenses is brought into coincidence on an image detector (128) and caused to interfere thereon by optical path length adjustment (138).

La présente invention concerne un procédé et un dispositif de microscopie optique tridimensionnelle mettant en oeuvre une paire de lentilles d'objectif (70, 72) disposées de part et d'autre d'un échantillon (94) et un éclairage non cohérent renforcé (84) pour obtenir une résolution renforcée en profondeur. Les lumières observées en provenance des deux lentilles d'objectif sont mises en coïncidence sur un détecteur d'image (128) et amenées à y entrer en interférence par un réglage de la longueur du trajet optique (138).

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