Method and apparatus for use in biological testing

Details Method and apparatus for use in biological testing Method and apparatus for use in biological testing

G - Physics

01

N

195/141, 150/19

G01N 27/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 1301102

ABSTRACT A method and apparatus for use in biological testing. The apparatus comprises electrophoresis apparatus comprising a tank (1) the interior of which is formed with a raised portion (5) which splits the tank into two compartments (6,7) containing electrophoresis electrolyte. Electrodes (8) are provided in each compartment, Electrophoresis is carried out through a gel (not shown) supported by a rigid porous plate (11). The gel is cast onto the porous plate with a transfer membrane (not shown) between the porous plate and the gel. Further electrodes (20,21) are mounted below and above the gel respectively. The method of the invention comprises a first stage using the electric field produced by the electrodes (8) to draw the samples through the gel and a second stage using the electric field produced by the electrodes (20,21) to draw the distributed macromolecules onto the transfer membrane. Figure 2

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