Method and apparatus of quantitative assays

C - Chemistry – Metallurgy – 12 – M

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C12M 1/34 (2006.01)

Patent

CA 2564940

A sample testing apparatus (100) for use in measuring an analyte in a sample (105) is disclosed. The apparatus includes a sample well (104) for receiving the sample. A solid analyte binding agent (160) is disposed in the sample well. The apparatus further 5 includes a mixing device (156) for mixing the sample with the solid analyte binding agent and a valve (129). The valve is configurable between a closed position for impeding flow of the sample from the sample well and an open position for releasing the sample from the sample well. The apparatus additionally includes a membrane (118) for receiving the sample once released from the sample well, the membrane having a capture reagent (164) 10 adapted to interact with at least one of the analyte, the analyte binding agent, or a complex thereof, to aid in determining the amount of the analyte in the sample.

L'invention concerne un appareil (100) pour tester un échantillon destiné à être utilisé dans la mesure d'un analyte dans un échantillon (105). L'appareil comprend une cupule d'échantillon (104) destinée à recevoir l'échantillon. Un agent de liaison (160) d'analyte solide est disposé dans la cupule. Ledit appareil comprend, de plus, un dispositif de mélange (156) destiné à mélanger l'échantillon avec l'agent de liaison d'analyte solide et une soupape (129). Ladite soupape peut être configurée entre une position fermée destinée à empêcher l'écoulement de l'échantillon de la cupule d'échantillon et une position ouverte destinée à libérer l'échantillon de la cupule d'échantillon. L'appareil comprend, de plus, une membrane (118) destinée à recevoir l'échantillon une fois libéré de la cupule, ladite membrane présentant un réactif (164) de capture conçu pour interagir avec au moins l'analyte, l'agent de liaison de l'analyte, ou un de leurs complexes, ce qui aide à déterminer la quantité de l'analyte dans l'échantillon.

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