Method and apparatus to determine paraffinophilic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) C12M 1/00 (2006.01) C12M 1/16 (2006.01)

Patent

CA 2237166

A method of determining the presence of a paraffinophilic microorganism in a specimen taken from a patient. The method includes providing a receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of the patient. The method then further includes inoculating the solution with the specimen and then placing in the receptacle a paraffin coated slide to bait the paraffinophilic microorganism. The slide is then analyzed after exposure to the specimen to determine the presence or absence of the paraffinophilic microorganism. An associated apparatus is also disclosed.

Cette invention concerne un procédé permettant de déterminer la présence d'un micro-organisme paraffinophile dans un échantillon prélevé chez un patient. Ce procédé consiste à mettre une solution aqueuse dans un ou plusieurs récipients, et à ajuster cette solution de manière à imiter les conditions cliniques in vivo du patient. Ce procédé consiste ensuite à inoculer l'échantillon dans la solution, et à placer dans le récipient une lame enduite de paraffine afin d'appâter le micro-organisme paraffinophile. On analyse ensuite la lame, après son exposition à l'échantillon, afin de déterminer la présence d'un micro-organisme paraffinophile ou l'absence de ce dernier. Cette invention concerne également un appareil associé à ce procédé.

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