Method and apparatus using a surface-selective nonlinear...

G - Physics – 01 – N

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G01N 33/543 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 21/84 (2006.01) G06F 17/00 (2006.01) G06F 19/00 (2006.01) H04L 29/08 (2006.01)

Patent

CA 2434076

A surface-selective nonlinear optical technique, such as second harmonic or sum frequency generation, is used to detect target-probe binding reactions or their effects, at an interface, without the use of labels. In addition, the direction of the nonlinear light is scattered from the interface in a well- defined direction and therefore its incidence at a detector some distance from the interface may be easily mapped to a specific and known location at the interface.

L'invention concerne une technique optique non linéaire, sélective en fonction de la surface, du type à génération de fréquence somme ou harmonique 2, permettant de déceler les interactions sonde-cible ou leurs effets, au niveau d'une interface, sans recourir à des marqueurs. Par ailleurs, la lumière non linéaire est diffusée depuis l'interface selon une direction bien définie, moyennant quoi il est aisé d'établir une correspondance entre l'incidence de ladite lumière au niveau d'un détecteur placé à une certaine distance de l'interface, d'une part, et un point spécifique déterminé de l'interface, d'autre part.

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