Method and apparatus using a surface-selective nonlinear...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12M 1/34 (2006.01) G01N 21/55 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2430430

A surface-selective nonlinear optical technique, such as second harmonic or sum frequency generation, is used to detect reactions between surface-attached probes and labeled targets or used to perform imaging of a surface. The surface-selective optical technique allows detection of only those target components near the interface while ignoring those present in the sample bulk. In addition, the direction of the nonlinear light is scattered from the surface in a well-defined direction and because of this, its incidence at a detector some distance from the surface may be easily mapped to a specific and known location on the surface.

L'invention concerne une technique optique non linéaire sélective en surface, par exemple génération de la deuxième harmonique ou de la fréquence-somme, utilisée pour détecter des réactions entre des sondes fixées aux surfaces et des cibles étiquetées, ou pour réaliser l'imagerie d'une surface. Ladite technique permet de détecter uniquement les composants cibles à proximité de l'interface et d'ignorer ceux présents dans la masse de l'échantillon. En outre, l'orientation de la lumière non linéaire est diffusée de la surface dans une direction bien définie et, de ce fait, son incidence sur le détecteur à quelque distance de la surface peut être aisément mise en correspondance dans un emplacement spécifique et connu sur la surface.

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