Method and assay involving improved specific binding...

Details Method and assay involving improved specific binding... Method and assay involving improved specific binding...

G - Physics

01

N

G01N 33/53 (2006.01) G01N 1/28 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2136757

A method of improving the specific binding reactivity of a polypeptide which is capable of polypeptide complexing is disclosed. The method involves contacting the polypeptide with an anionic surfactant capable of decomplexing the polypeptide under suitable conditions resulting in at least some decomplexing of the polypeptide. The polypeptide is then contacted with a nonionic surfactant capable of complexing with the anionic surfactant under suitable conditions resulting in at least some of the decomplexed polypeptide remaining decomplexed. A method of assay of a sample containing a polypeptide which is capable of polypeptide complexing is also disclosed.

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