Method and associated materials for measuring glucose level...

G - Physics – 01 – N

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150/2, 324/28, 1

G01N 33/66 (2006.01) A61K 49/00 (2006.01)

Patent

CA 1206856

ABSTRACT A method for measuring the level of glucose in animal body fluids such as blood and urine, comprises contacting the body fluid with a glucose indicator comprising a reversible complex of a carbohydrate component, a binding macromolecular component, and an indicator element bound to one of the com- ponents, and maintaining the body fluid in contact with the indicator for a period of time sufficient to permit any glu- cose present in the body fluid to displace the carbohydrate component in the complex, and to thereby release the indicator element to signify the presence of glucose. Preferably, the macromolecular component comprises one or more lectins; and the carbohydrate component may be one or more of the sugars for which testing is desired. In one embodiment of the present invention, the indicator may comprise a dye or color-forming radical, associated with either the lectin or the carbohydrate, incorporated in a test strip or kit, for rapid, in vitro glucose level measurement. Alternately, the indicator may be a glucose monitor comprising an electrode adapted for in vivo measurement of glucose con- centration, by implantation in registry with the body fluid system of the animal for which measurement is desired.

397815

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