Method and cell line for continuous production of...

C - Chemistry – Metallurgy – 12 – N

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195/1.36

C12N 7/00 (2006.01)

Patent

CA 1266243

ABSTRACT A cell system is disclosed for the reproducible detection and isolation of human T-lymphotropic retro- viruses (HTLV-family) with cytopathic effects (HTLV-III) from patients with the acquired immune deficiency syn- drome (AIDS), pre-AIDS and healthy carriers. One neo- plastic aneuploid T-cell line derived from an adult with lymphoid leukemia, termed HT, was susceptible to infec- tion with HTLV-III, which is transformed and provides T- cell populations which are highly susceptible to and permissive for HTLV-III, and convenient for large scale production, isolation, and biological detection of the virus. Other operational neoplastic T-cell lines which are positive for OKT4 marker, e.g., Molt 3, CEM, Ti7.4 and HUT78, can produce HTLV-III in a large amount and retain its unlimited capability for growth.

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