Method and cell line for obtaining plasminogen activators

C - Chemistry – Metallurgy – 12 – N

Patent

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195/123, 195/34.

C12N 9/64 (2006.01) C12N 5/00 (2006.01) C12N 9/72 (2006.01)

Patent

CA 1267621

ABSTRACT OF THE DISCLOSURE A method of increasing the yield of plasminogen activator from a plasminogen activator-producing cell line by adapting said cell line to grow in a suitable growth medium essentially free of fetal calf serum is disclosed. Also disclosed are cell lines capable of producing relatively large quantities of plasminogen activator, and a chemically defined growth medium suitable for adapting a plasminogen activator- secreting cell line to growth in the absence of fetal calf serum. MS-1338-CIP

477723

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