Method and cells to screen combinatorial libraries

Details Method and cells to screen combinatorial libraries Method and cells to screen combinatorial libraries

G - Physics

01

N

G01N 33/48 (2006.01) C12N 11/16 (2006.01) C12N 15/10 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2310381

This invention is a method for identifying and isolating at least one of the following: a polypeptide of interest and/or a gene thereof; a target compound and/or a gene thereof, wherein the polypeptide of interest, or the target compound, or both are unknown. The method is based on the steps of: (1) displaying a library of a polypeptide of interest on the surface of a population of prokaryotic or eukaryotic cells; (2) exposing the population to a target compound; and (3) exposing the population to a selection agent. Interaction among the three components "imprints" the polypeptide: that is, the interaction selects for those cells in which the polypeptide interacts with the target compound in a prescribed manner.

Cette invention se rapporte à un procédé destiné à identifier et à isoler au moins un des composants suivants: un polypeptide d'intérêt et/ou un gène de ce polypeptide: un composé cible et/ou un gène de ce composé, le polypeptide d'intérêt, le composé cible, ou bien les deux à la fois étant inconnus. Le procédé consiste à: (1) afficher une bibliothèque de polypeptides d'intérêt sur la surface d'une population de cellules procaryotes ou eucaryotes; (2) exposer cette population à un composé cible; et (3) exposer ladite population à un agent sélecteur. L'interaction des trois composants "grave" le polypeptide: en d'autres termes, cette interaction a pour effet de sélectionner les cellules dans lesquelles le polypeptide interagit avec le composé cible de la manière prescrite.

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