Method and composition for analyzing a carbohydrate polymer

Details Method and composition for analyzing a carbohydrate polymer Method and composition for analyzing a carbohydrate polymer

G - Physics

01

N

G01N 33/53 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2407272

Disclosed is a method for characterizing a carbohydrate polymer by identifying at least two binding agents that bind to the carbohydrate polymer. Binding is preferably determined by contacting the carbohydrate polymer with substrate that contains a plurality of first saccharide-binding agents affixed at predetermined locations on the substrate. The carbohydrate polymer is allowed to contact the substrate under conditions that allow for formation of a first complex between the first saccharide-binding agent and the carbohydrate polymer. A second saccharide-binding agent, which preferably includes a label, is also contacted with the carbohydrate polymer under conditions that allow for formation of a second complex between the second binding agent and the first complex. Identification of the first and second binding agent allows for characterization of the polysaccharide.

L'invention concerne un procédé permettant de caractériser un polymère glucidique par identification d'au moins deux agents de liaison se liant au polymère glucidique. De préférence, la liaison est déterminée par mise en contact du polymère glucidique avec un substrat contenant plusieurs premiers agents de liaison à la saccharide fixés à des emplacements prédéterminés sur le substrat. Le polymère glucidique peut ainsi entrer en contact avec le substrat dans des conditions permettant la formation d'un premier complexe entre ledit premier agent de liaison et le polymère glucidique. Un second agent de liaison à la saccharide, comprenant, de préférence, un traceur, est également mis en contact avec le polymère glucidique dans des conditions permettant la formation d'un second complexe entre le second agent de liaison et le premier complexe. L'identification du premier agent de liaison et du second agent de liaison permet de caractériser le polysaccharide.

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