Method and composition for detecting bacterial contamination...

Details Method and composition for detecting bacterial contamination... Method and composition for detecting bacterial contamination...

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01) C12Q 1/04 (2006.01) C12Q 1/06 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2223451

This invention relates to a method for detecting the existence or measuring the concentration of total viable bacteria in a test sample from a food product. A medium is provided which contains three or more different enzyme substrates each having a nutrient moiety and a detectable moiety linked together. When a substrate is hydrolysed by a bacterial enzyme to create a separate detectable moiety, it causes or produces a detectable signal. These substrates produce detectable signals when any one of a phosphatase enzyme, a glycosidase enzyme or a peptidase enzyme is present in the medium.

Cette invention se rapporte à un procédé de détection ou de mesure de la totalité de la concentration de bactéries vivantes dans un échantillon d'essai prélevé dans un produit alimentaire. L'invention porte également sur un milieu contenant au moins trois substrats enzymatiques différents, chacun possédant une fraction nourricière et une fraction détectable qui sont liées l'une à l'autre. Lorsqu'un substrat est hydrolysé par une enzyme bactérienne pour créer une fraction détectable séparée, il suscite ou génère un signal détectable. Ces substrats génèrent des signaux détectables lorsque n'importe laquelle des enzymes phosphatase, glycosidase ou peptidase est présente dans le milieu.

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