Method and composition for direct determination of iron in...

G - Physics – 01 – N

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150/11

G01N 33/52 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1131114

Abstract A direct method is disclosed for determination of iron in blood serum comprising reacting the blood serum with ferrozine in the presence of a reducing agent to bring the iron ions into divalent form, thiosemicarbazide and hydrochloric acid at a pH from 1.7 to 2.1, without any addition of buffers or tensioactive agents and determining colorimetrically the iron content of the specimen by means of the colored complex formed between iron ion and ferrozine against a reagent blank.

339417

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