Method and composition for enhancing the activity of an enzyme

Details Method and composition for enhancing the activity of an enzyme Method and composition for enhancing the activity of an enzyme

C - Chemistry, Metallurgy

11

D

C11D 3/386 (2006.01) C11D 3/26 (2006.01) C11D 3/30 (2006.01) C11D 3/34 (2006.01) C11D 3/39 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2369127

There is provided a process for enhancing the activity of a phenol oxidizing enzyme, comprising adding to the enzyme, as an enhancer for the activity of said enzyme, one or more compounds having the formula (I) or (II) wherein:A is an optionally substituted electron-withdrawing group, chosen from the group consisting of nitrile, carboxyl, and esters and salts thereof, amides, aldehydes, ketones, sulfoxides, sulfones, or sulphonates, and B is an optionally substituted electron-donating group, chosen from the group consisting of the oxide anion, sulfide anion, oxides, amines, imines, hydroxides, sulfides, ethers, carboxylic acids, and halogen substituents, and R1-R5 may each independently represent hydrogen, hydroxy, halogen, nitroso, formyl, carboxyl, and esters and salts thereof, carbamoyl, sulfo, and esters and salts hereof, sulfamoyl, nitro, amino, phenyl, C1-C20 alkyl, C1-C8 alkoxy, carbonyl-C1-C6-alkoxy, aryl-C1-C6-alkyl, and R2 and R5 together may form an alkylene group or an alkenylene group.

La présente invention concerne un procédé permettant d'améliorer l'activité d'une enzyme d'oxydation phénolique. Ce procédé consiste à ajouter à l'enzyme, en tant qu'agent d'amélioration d'activité de ladite enzyme, un ou plusieurs composés de formule (I) ou (II), dans lesquelles A est un groupe récepteur d'électron, éventuellement substitué, choisi dans le groupe constitué par le nitrile, le carboxyle, les esters et sels de ces derniers, les amides, les aldéhydes, les cétones, les sulfoxydes, les sulfones ou les sulfonates, et B est un groupe donneur d'électron, éventuellement substitué, choisi dans le groupe constitué par l'anion oxyde, l'anion sulfure, les oxydes, les amines, les imines, les hydroxydes, les sulfures, les éthers, les acides carboxyliques et les substituants halogénés, et R¿1?-R¿5? peuvent représenter indépendamment hydrogène, hydroxy, halogène, nitroso, formyle, carboxyle, carbamoyle, sulfo, esters et sels de ces derniers, sulfamoyle, nitro, amino, phényle, alkyle C¿1?-C¿20?, alkoxy C¿1?-C¿8?, alkoxy C¿1?-C¿6? carbonyle, alkyle C¿1?-C¿6? aryle, et R¿2? et R¿5? peuvent former ensemble un groupe alkylène ou un groupe alkénylène.

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