Method and composition for isolating white cell elements

G - Physics – 01 – N

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G01N 33/577 (2006.01) A61K 35/14 (2006.01) B01D 43/00 (2006.01) G01N 33/569 (2006.01) G01N 33/80 (2006.01)

Patent

CA 1228025

53-74F/UC4.20 METHOD AND COMPOSITION FOR ISOLATING WHITE CELL ELEMENTS Abstract of the Disclosure A composition of matter and method for using the composition of matter to isolate various cell elements from a heterogeneous cell suspension in a simple one step procedure. The composition is a monoclonal antibody mixture including complement and a non-toxic density gradient media. The composition provides a negative selection technique in which undesired subpopluation cells are lysed and separated from the desired cell subpopulation. The invention has particular application to isolation of white cell elements.

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