C - Chemistry – Metallurgy – 09 – B
Patent
C - Chemistry, Metallurgy
09
B
150/3, 260/396,
C09B 11/04 (2006.01) G01N 33/52 (2006.01)
Patent
CA 1340123
A method and composition for the assay for albumin at an essentially neutral to an alkaline pH. The method utilizes indicator dyes that, when sufficiently buffered in a pH range of approximately 6 to approximately 8, allows the detection of albumin in urine or serum. The indicator dyes utilized in the method of the present invention have the general formula: (see fig. i) or (see fig. II) wherein R1 and R2 are substituted aromatic heterocyclic or carbocyclic moieties having one or more electron-withdrawing functionalities present on the aromatic ring; R3 is a substituted aromatic heterocyclic or carbocyclic moiety having one or more electron-withdrawing functionalities present on the aromatic ring, or R3 is a non-aromatic electron-withdrawing moiety; R4 is a substituted quasi-aromatic quinone-like heterocyclic or carbocyclic moiety having one or more electron-with-drawing functionalities present on the quasi-aromatic quinone-like ring; and R5 is a substituted aromatic heterocyclic or carbocyclic moiety having one or more electron-withdrawing functionalities, including at least one hydroxy functionality, present on the aromatic ring.
606527
Miles Inc.
Osler Hoskin & Harcourt Llp
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