Method and composition to evaluate cytochrome p450 2d6...

A - Human Necessities – 61 – K

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A61K 51/00 (2006.01) C12Q 1/00 (2006.01) G01N 33/50 (2006.01) C12N 9/02 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2606136

The present invention relates, generally to a method of determining and assessing cytochrome P450 2D6 isoenzyme (CYP2D6)-related metabolic capacity in an individual mammalian subject via a breath assay, by determining the relative amount of 13CO2 exhaled by a the subject upon intravenous or oral administration of a 13C-labeled CYP2D6 substrate compound. The present invention is useful as an in vivo phenotype assay for evaluating CYP2D6- related activity using the metabolite 13CO2 in expired breath and to determine the optimal dosage and timing of administration of CYP2D6 substrate compound.

La présente invention se rapporte, de manière générale, à un procédé visant à déterminer et à évaluer la capacité métabolique liée à l~isoenzyme 2D6 du cytochrome P450 (CYP2D6) chez un mammifère au moyen d~un test respiratoire, en déterminant la quantité correspondante de ¿13?CO?2#191 exhalée par le sujet à qui l~on administre un substrat CYP2D6 étiqueté ¿13?C par voie orale ou intraveineuse. La présente invention est utile dans les essais phénotypiques in vivo à la fois pour évaluer l~activité liée au CYP2D6 en utilisant le métabolite ¿13?CO?2#191 dans le souffle expiré et pour déterminer le dosage optimum du substrat CYP2D6 et le moment opportun pour l~administrer.

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