Method and compositions for improving allogeneic...

A - Human Necessities – 61 – K

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A61K 35/14 (2006.01) A61K 35/28 (2006.01) C12N 5/06 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2351194

Methods of reducing or preventing graft versus host disease in an allogeneic hematopoietic system reconstituting cells transplant recipient comprising administering to the recipient allogeneic hematopoietic system reconstituting cells in which the number of dendritic cells has been effectively reduced, thereby reducing or preventing graft versus host disease, are provided. Also provided are methods of increasing the chance of survival for an allogeneic hematopoietic system reconstituting cells transplant recipient comprising administering to the recipient allogeneic hematopoietic system reconstituting cells in which the number of dendritic cells has been effectively reduced, thereby improving the chance for survival of the recipient. Columns for preparing hematopoietic system reconstituting cells prior to their transplantation are disclosed that provide for the selection of CD34+ cells and the removal of dendritic cell progenitors.

Ces méthodes visant à réduire ou à empêcher une réaction du greffon contre l'hôte chez un receveur ayant subi une transplantation cellulaire allogénique destinée à reconstituer le système hématopoïétique consistent à administrer au receveur un ensemble de cellules reconstituant le système hématopoïétique, ensemble dans lequel le nombre de cellules dendritiques a été effectivement diminué, ce qui permet de réduire ou d'empêcher une réaction du greffon contre l'hôte. L'invention a également trait à des techniques visant à augmenter les chances de survie d'un receveur de transplantation cellulaire allogénique destinée à reconstituer le système hématopoïétique, consistant à administrer à ce patient receveur un ensemble de cellules reconstituant le système hématopoïétique dans lequel le nombre de cellules dendritiques a été effectivement diminué, ce qui permet d'augmenter ses chances de survie. L'invention concerne également des colonnes servant à la préparation de cellules de reconstitution du système hématopoïétique avant leur transplantation et permettant une sélection de cellules CD34+ ainsi que l'enlèvement de précurseurs de cellules dendritiques.

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