Method and compositions for nucleic acid amplification

Details Method and compositions for nucleic acid amplification Method and compositions for nucleic acid amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2692092

The present teachings provide methods, compositions, and kits for nucleic acid amplification. In some embodiments of the present teachings, amplification reactions are performed with at least one high stability primer. In some embodiments, the present teachings provide a method comprising a high stability primer for amplification of a nucleic acid sequence in a sample comprising a target nucleic acid sequence and a PCR inhibitor.

La présente invention concerne des procédés, des compositions et des kits pour l'amplification d'acides nucléiques. Selon certains modes de réalisation de la présente invention, les réactions d'amplification sont réalisées avec au moins une amorce de grande stabilité. Selon certains modes de réalisation, la présente invention concerne un procédé qui comprend une amorce de grande stabilité pour l'amplification d'une séquence d'acides nucléiques dans un échantillon qui comprend une séquence d'acides nucléiques cibles et un inhibiteur de PCR.

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