Method and compositions useful for the activation of silent...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/00 (2006.01) C12N 15/52 (2006.01) C12N 15/62 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2311632

Compositions and method for inducing expression of a desired DNA sequence in a stable transformed plant expressing a hybrid transcription factor, comprising a fusion of the DNA-binding domain and a transcription activation domain, which is an effector of expression of the desired DNA sequences controlled by a synthetic promoter, said synthetic promoter preferably comprising concatemeric copies of the cis-acting site recognized by DNA-binding domain of the hybrid transcription factor, fused to a promoter.

L'invention porte sur des compositions et procédés provoquant l'expression d'une séquence d'ADN désirée dans une plante transformée stable, exprimant un facteur hybride de transcription et consistant en une fusion du domaine de fixation de l'ADN et du domaine d'activation de transcription, lequel est un effecteur de l'expression des séquences d'ADN désirées régi par un promoteur de synthèse fait de préférence de copies concatémères du site à comportement cis, reconnu par le domaine de fixation de l'ADN du facteur hybride de transcription fusionné en promoteur.

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