Method and device for analysing chemical or biological samples

B - Operations – Transporting – 01 – L

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Details

B01L 3/00 (2006.01) B01J 19/00 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01) G01N 21/77 (2006.01)

Patent

CA 2424806

The invention relates to a method and related device for analysing chemical or biological samples. Chemical or biological samples and/or targets (probes) are applied to an outer cylindrical lateral area of a carrier in the form of individual defined spots, or are loaded into bore holes in the form of liquid drops, said bore holes being recessed in the lateral area of the carrier. The carrier is introduced into a recess in the holder, said recess being essentially complementary to the cylindrical lateral area, the samples and/or targets are influenced by means of physical and/or chemical interactions, and the accordingly modified spots are then analysed. The invention also relates to the use of a novel carrier system for examining chemical or biological samples, whichcontrary to conventional planar biochips is characterised by a cylindrical geometry, whereby substances can be applied, immobilised for example, on the functionalised lateral area of the cylinder or in the radial bore holes recessed in the cylinder casing. An analysis system having clearly defined reaction volumes is implemented by co-operating with a complementary holder, said analysis system being easily standardised and highly automated.

L'invention concerne un procédé et un dispositif correspondant d'analyse d'échantillons chimiques ou biologiques. Ledit procédé consiste à appliquer des échantillons et/ou cibles (sondes) chimiques ou biologiques sous forme de points individuels définis sur une surface d'enveloppe cylindrique extérieure d'un support, ou sous forme de gouttelettes de fluide dans des perçages pratiqués dans la surface d'enveloppe du support. Ledit procédé consiste également à amener le support dans une cavité d'un élément de fixation essentiellement complémentaire à la surface d'enveloppe cylindrique, à influencer les échantillons et/ou cibles par l'intermédiaire d'interactions physiques et/ou chimiques, et à analyser les points ainsi modifiés. L'invention concerne également l'utilisation d'un système support destiné à l'étude d'échantillons chimiques ou biologiques, se caractérisant par une géométrie cylindrique contrairement aux biopuces planes habituelles, des substances pouvant être appliquées, par exemple immobilisées, sur la surface d'enveloppe fonctionnalisée du cylindre ou dans des perçages radiaux pratiqués dans l'enveloppe de cylindre. L'interaction avec un élément de fixation complémentaire permet de mettre en oeuvre un système d'analyse présentant des volumes de réaction définis de manière univoque, pouvant être standardisé simplement et automatisé largement.

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