A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
195/145, 47/4, 1
A01H 4/00 (2006.01) C12M 3/00 (2006.01)
Patent
CA 2037896
(57) Abstract The invention relates to a method for aseptically propagating cells and tissue, more particular for the vegetative propaga- tion of plantlets, whereby in a sterile environment starting material is placed in individual memberane capsules, into which an amount of nutrient medium for the material to be propagated or to be rooted in advance, upon which the capsules are sealed. Ac- cording to the invention a continuous capsule chain (2) is caused to gradually unwind from a flat folded storage (1) condition and to move in a substantially upright position along a number of stations, viz. a first station, (A) where the capsule chain passes through the space between two upright walls (12), while an underpressure is created within the latter space, which, under pressure, causes the opposing membrane walls of the capsule chain (2) to spread apart, after which the thus opened capsule cells are filled with a quantity of nutrient medium with the aid of filling means which may be introduced from above; a second station, (B) in which a plantlet to be propagated or rooted respectively is placed from above in each of the capsule cells which are previously provided with nutrient medium; a third station (24) in which the capsule cells containing culture material are closed by a horizon- tal seal and a fourth station, in which the filled and closed capsule chain is placed in a storage container in a horizontally packed condition.
Fetherstonhaugh & Co.
Permx B.v.
Van Oeveren Jacobus J.
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