Method and device for attachment of biologically useful...

C - Chemistry – Metallurgy – 12 – N

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195/35.3

C12N 11/00 (2006.01) C12N 11/06 (2006.01) G01N 33/543 (2006.01) G01N 33/547 (2006.01)

Patent

CA 1316858

ABSTRACT OF THE DISCLOSURE A method and kit for indirectly attaching a biologically useful material to a solid phase by means of a linking polymer is described. The linking polymer is selected from difunctional polyalkylene ether polymers wherein the difunctionality is at the distal and proximal ends of the polymer. The proximal end of the polymer is attached to the solid phase and the distal end of the polymer is joined to the material. Very low density of the linking polymer (less than 100 nanomoles per square meter) reduces interference with and increases the activity of the material. Enzymes and other active molecules or combinations of molecules are immobilized in the solid phase.

601224

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