G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 27/447 (2006.01) C07K 1/26 (2006.01)
Patent
CA 2424298
The invention relates to a method and device for separating complex protein mixtures with the aid of high-resolution two-dimensional electrophoresis (2D electrophoresis, 2DE) in large gels. The invention is characterized in that the separation of the proteins in the first dimension is carried out in hollow, porous and protein-permeable materials and, in the second dimension, the porous materials are placed inside a glass cassette without being modified. Said glass cassette contains a flat gel into which the proteins migrate through the wall of the porous material during electrophoresis. The 2DE, together with the mass spectrometry, forms the basic technique, e.g. for the proteomic analysis, i.e. for separating and identifying the entire protein of a cell type, organ or of an organism.
L'invention concerne un procédé et un dispositif permettant de séparer des mélanges de protéines complexes par électrophorèse bidimensionnelle à haute résolution (électrophorèse en 2D, 2DE) dans des gels importants. L'invention se caractérise en ce que dans la première dimension, la séparation des protéines s'effectue dans des matériaux poreux creux perméables aux protéines et en ce que dans la seconde dimension, les matériaux poreux sont introduits inchangés dans un coffret en verre contenant un gel plat dans lequel les protéines migrent à travers la paroi du matériau poreux pendant électrophorèse. La 2DE constitue conjointement avec la spectrométrie de masse la technique de base par ex. pour l'analyse protéomique, c.-à-d. pour la séparation et l'identification de la protéine globale d'un type cellulaire, d'un organe ou d'un organisme.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Protagen Ag
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