Method and device for carrying out biochemical reactions...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/40 (2006.01) B01J 19/00 (2006.01) B01J 19/24 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2340712

Device, method and kits for carrying out biochemical reactions, in particular for cell-free polypeptide biosynthesis composed of an external housing which encloses an inner housing with incorporated wells and a supply chamber, wherein the wells of the inner housing each contain a producing system during the biochemical reaction, the supply chamber contains a supply liquid during the biochemical reaction and the wells of the inner housing and the supply chamber are separated by a semipermeable membrane characterized in that the inner housing has at least two wells the lower ends of which are closed by a semipermeable membrane and the upper ends of which protrude from the supply liquid contained in the supply chamber, and is connected to means for moving and incubating the producing systems and to the supply liquid.

La présente invention concerne un dispositif, un procédé et un équipement destinés à la réalisation de réactions biochimiques, en particulier pour la biosynthèse polypeptidique sans cellule. Le dispositif comprend une enveloppe externe qui recouvre une enveloppe interne dans laquelle sont formées des cavités et un compartiment de distribution. Durant la réaction biochimique, les cavités de l'enveloppe interne comprennent chacune un système de production, le compartiment de distribution contient un liquide de distribution, et les cavités de l'enveloppe interne sont séparées du compartiment de distribution par une membrane semi-perméable. L'invention se caractérise en ce que l'enveloppe présente au moins deux cavités dont les extrémités inférieures sont fermées par la membrane semi-perméable et dont les extrémités supérieures dépassent du liquide contenu dans le compartiment de distribution, et en ce que le dispositif est relié à des éléments permettant le mouvement et l'équilibrage de température des systèmes de production ainsi que du liquide de distribution.

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