Method and device for characterising and/or for detecting a...

G - Physics – 01 – N

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G01N 33/53 (2006.01) C12Q 1/68 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2418861

The invention relates to a method for characterising and/or for detecting a bonding complex comprising the following steps: providing a first bonding partner and a conjugate from a second and a third bonding partner and providing a fourth bonding partner; forming a link between the bonding partners, wherein the first bonding partner combined with the second bonding partner forms a sample complex and the third bonding partner combined with the fourth bonding partner forms a reference complex; applying a force to the link, which results in the separation of the sample complex or reference complex; determing which of the bonding complexes were separated.

La présente invention concerne un procédé de caractérisation et/ou d'identification d'un complexe de liaison comprenant les étapes suivantes: mise à disposition d'un premier partenaire de liaison et d'un conjugué constitué d'un deuxième et d'un troisième partenaire de liaison, et mise à disposition d'un quatrième partenaire de liaison; formation d'un enchaînement des partenaires de liaison, le premier partenaire de liaison formant un complexe échantillon avec le deuxième partenaire de liaison, et le troisième partenaire de liaison formant un complexe de référence avec le quatrième partenaire de liaison; application d'une force sur la chaîne, ladite force conduisant à la séparation du complexe échantillon ou du complexe de référence; et détermination duquel des deux complexes a subit la séparation.

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