Method and device for creating micro-arrays

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 60/14 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) C12M 1/14 (2006.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01) C40B 50/00 (2006.01) G01N 1/31 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2425634

A method and device are provided for simultaneously creating a plurality of identical micro-arrays of biological samples. The invention utilizes a plurality of substrates, each of which having a top side, a bottom side, and a pattern of through-holes. Each through-hole has a wider upper cross-section, a narrower lower cross-section, and a step or plateau formed in the transition area. When the substrates are stacked, through-holes are in registry and form tunnels extending through the stack of substrates. Reagents of interest are caused to flow through the tunnels and deposit on the step or plateau area. A barrier layer may be provided to prevent leak-through between neighboring holes. After the desired reagents have been deposited, the substrates are separated. In this manner a series of micro-arrays, each capable of containing hundreds or thousands of biological samples such as cDNA fragments, is formed simultaneously.

L'invention concerne un procédé et un dispositif permettant de créer simultanément plusieurs micro-réseaux identiques d'échantillons biologiques. Elle concerne plusieurs substrats, chacun présentant un côté supérieur et un côté inférieur et un motif de trous de passage. Chaque trou de passage présente une section transversale supérieure large et une section transversale inférieure étroite, ainsi qu'un niveau ou un plateau formé dans la zone de transition. Lorsque les substrats sont empilés, les trous de passage sont en communication et forment des tunnels traversant la pile de substrats. Les réactifs d'intérêt traversent les tunnels et se déposent dans la zone du niveau ou du plateau. On peut installer une couche barrière pour empêcher des fuites entre des trous voisins. Une fois les réactifs voulus déposés, on sépare les substrats. Ainsi, on peut former simultanément une série de micor-réseaux capables de contenir chacun des centaines, voire des milliers, d'échantillons biologiques, tels que des fragments d'ADNc.

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