Method and device for culturing live cells by coupling a...

C - Chemistry – Metallurgy – 02 – F

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C02F 3/34 (2006.01) C02F 3/00 (2006.01)

Patent

CA 2539478

The invention relates to a method for continuously, semi-continuously or discontinuously treating a substrate (24) consisting in placing said substrate in a bioreactor receiver (1) and in exposing it to the action of a living cell culture (C1) which makes it possible to carry out a reaction (R1) on the substrate (24) and to which a medium is periodically inoculated with the aid of living cells (C2) improving said reaction. Said living cells (C2) are obtainable by selection from a population of dynamic living cells carried out by an automatic selection device (2) which is supplied by the same substrate (24) as the bioreactor receiver (1) and is originally inoculated with the living cells (C1) contained in the bioreactor receiver (1) tank and an operating device.

Procédé de traitement en continu, semi-continu ou discontinu d'un substrat (24), dans lequel ledit substrat (24) installé dans un récipient bioréacteur (1) est soumis à l'action d'une culture de cellules vivantes C1 permettant d'effectuer une réaction R1 sur ledit substrat (24) et dans lequel on inocule périodiquement le milieu à l'aide de cellules vivantes C2 améliorant ladite réaction, lesdites cellules vivantes C2 étant issues d'une sélection effectuée par un dispositif de sélection automatique (2), d'une population de cellules vivantes dynamiques et ledit dispositif de sélection automatique (2) de cellules vivantes étant alimenté par le même substrat (24) que le récipient bioréacteur (1) et étant inoculé à l'origine par les cellules vivantes C1 présentes dans la cuve du récipient bioréacteur (1) et dispositif de mise en oeuvre.

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