Method and device for detecting or measuring the amount of a...

Details Method and device for detecting or measuring the amount of a... Method and device for detecting or measuring the amount of a...

G - Physics

01

N

G01N 33/566 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2161650

The invention is directed to methods and a device for detecting or measuring the amount of a cell-associated molecule in a biological fluid sample. Cell-associated molecules which can be detected or measured include, but are not limited to, cell surface antigens, intracellular cytokines, microbial antigens, pharmacological agents or their metabolites, and nucleic acids. In the preferred embodiment, a sample of whole blood is collected on filter paper and dried. A dot is punched from the dried blood spot and the analyte of interest eluted from the paper with a buffered, relatively highly concentrated detergent solution. The eluate is then assayed in a standard assay, such as an immunoassay.

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