Method and device for detection of nucleic acid or analyte...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) B01L 7/00 (2006.01) C12M 1/34 (2006.01) C12M 1/38 (2006.01) C12M 1/40 (2006.01) C12Q 1/70 (2006.01) G01N 21/55 (2006.01)

Patent

CA 2133643

2133643 9320240 PCTABS00027 An apparatus and method for detecting amplified target nucleic acid is provided wherein the presence and concentration of amplified target is determined by total internal reflection over the course of the amplification reaction. A method and apparatus for detecting target nucleic acid is also provided wherein the presence and concentration of target is determined by total internal reflection and coupling of the target to the TIR element by scissile linkage. An improved immunoassay using total internal reflection and differential temperature cycling is further provided.

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