Method and device for determining the volume of a liquid sample

G - Physics – 01 – F

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G01F 22/00 (2006.01) B01L 99/00 (2010.01) B01L 3/02 (2006.01) G01F 25/00 (2006.01) G01N 21/77 (2006.01) G01N 30/00 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2363475

The invention concerns a method, a device, and a test kit for performing this method for determining the volume of a sample of the liquid (A). In the method -- to stain the liquid (A) -- a specific concentration of a chromophoric indicator is provided in this liquid (A), a sample is separated from the liquid (A), the optical absorption of the separated sample is measured, and the volume of the sepa- rated sample is determined by correlation of the measured optical absorption with the concentration of indicator in this liquid (A). The method is characterized in that ions which generate the color of the sample by complexing with a specific ligand are used as the indicator to stain the liquid (A).

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