Method and device for evaluating fluorescence image records

A - Human Necessities – 61 – B

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A61B 5/00 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2691555

The invention relates to a method and a device for evaluating fluorescence partial images representing the same object region, independently of stray light. Two partial images of the objects are produced in the red and in the green by a camera (10). A quotient image is produced in pixels from said two partial images, and the frequency of the occurrence of the image points having a pre-defined red/green ratio is determined for said quotient image. The mean values and the width are determined for the distribution curve obtained in this way. The two end variables of the distribution curve are used to calculate a threshold value. The quotient image is modified using said threshold value such that its contrast in relation to interesting details is increased.

L'invention concerne un procédé et un dispositif d'évaluation d'images partielles de fluorescence représentant une même zone d'un objet, indépendamment de la lumière parasite. Deux images partielles de l'objet sont réalisées dans le rouge et dans le vert au moyen d'une caméra (10). Une image de quotient est générée sur la base de pixels à partir de ces deux images partielles et la fréquence d'occurrence de pixels présentant un rapport rouge/vert prescrit est déterminée pour cette image de quotient. Une moyenne et une largeur sont déterminées pour la courbe de répartition ainsi obtenue. Ces deux grandeurs finales de la courbe de répartition servent au calcul d'une valeur seuil. Cette valeur seuil sert ensuite à modifier l'image de quotient de manière à augmenter son contraste pour des détails pertinents.

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